Fluorescence microscopy three-dimensional depth variant point spread function interpolation using Zernike moments
نویسندگان
چکیده
منابع مشابه
Fluorescence microscopy three-dimensional depth variant point spread function interpolation using Zernike moments
In three-dimensional fluorescence microscopy the point spread function (PSF) changes with depth, inducing errors in the restored images when these variations are neglected during the deconvolution of thick specimens. Some deconvolution algorithms have been developed to take the depth variations of the PSF into consideration. For these algorithms, the accuracy of the estimated structures depends...
متن کاملEnhanced three-dimensional deconvolution microscopy using a measured depth-varying point-spread function.
We present a technique to systematically measure the change in the blurring function of an optical microscope with distance between the source and the coverglass (the depth) and demonstrate its utility in three-dimensional (3D) deconvolution. By controlling the axial positions of the microscope stage and an optically trapped bead independently, we can record the 3D blurring function at differen...
متن کاملImproving Accuracy of Pseudo Zernike Moments using Image Interpolation
Pseudo Zernike Moments (PZMs) are very popular moments among the family of orthogonal radial moments. While several methods have been proposed to enhance accuracy, accurate PZMs computation for gray level images is still an open issue. PZMs suffer from image discretization error, geometric error and numerical integration error, which result in the degradation of the reconstructed images for hig...
متن کاملThree-dimensional holographic fluorescence microscopy.
Most commonly used methods for three-dimensional (3D) fluorescence microscopy make use of sectioning techniques that require that the object be physically scanned in a series of two-dimensional (2D) sections along the z axis. The main drawback in these approaches is the need for these sequential 2D scans. An alternative approach to fluorescence imaging in three dimensions has been developed tha...
متن کاملSelf-interference fluorescence microscopy: three dimensional fluorescence imaging without depth scanning
We present a new method for high-resolution, three-dimensional fluorescence imaging. In contrast to beam-scanning confocal microscopy, where the laser focus must be scanned both laterally and axially to collect a volume, we obtain depth information without the necessity of depth scanning. In this method, the emitted fluorescence is collected in the backward direction and is sent through a phase...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of the Optical Society of America A
سال: 2011
ISSN: 1084-7529,1520-8532
DOI: 10.1364/josaa.28.001864